Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human CXCR4 protein to be purified and analysed in vitro.
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human CXCR4 protein has a MW of 39.7 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
ELISA plates were pre-coated with Synthetic Nanodisc Human CXCR4 Protein (A318433) (0.2 µg/well). Serial diluted Anti-CXCR4 Antibody [Ulocuplumab Biosimilar] - Azide free (A318864) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From this data, the EC50 for Anti-CXCR4 Monoclonal Antibody binding with CXCR4-Nanodisc is 2442 µg/ml.
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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