Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human CXCR3 protein to be purified and analysed in vitro.
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human CXCR3 protein has a MW of 40.5 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
C-X-C chemokine receptor type 3, CD183, CKR-L2, CXC-R3, CXCR-3, G protein-coupled receptor 9, GPR9, Interferon-inducible protein 10 receptor, IP-10 receptor
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA plates were pre-coated with Anti-CXCR3 Antibody [DM208] - BSA and Azide free (A318501) at 0.2µg/well. Serial diluted Synthetic Nanodisc Human CXCR3 Protein (A317349) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From the above data, the EC50 for Synthetic Nanodisc Human CXCR3 Protein (A317349) binding with Synthetic Nanodisc Human CXCR3 Protein (A317349) is 15.79ng/ml.
Western Blot - Synthetic Nanodisc Human CXCR3 Protein (A317349)
Western Blot analysis of Synthetic Nanodisc Human CXCR3 Protein (A317349) with Anti-Flag Monoclonal Antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution.
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