Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human Claudin18.2 protein to be purified and analysed in vitro.
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human Claudin18.2 protein has a MW of 27.5 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Synthetic Nanodisc Human Claudin18.2 Protein (A318483)
ELISA plates were added with Synthetic Nanodisc Human Claudin18.2 Protein (A318483) (0.2 µg/per well) on an Anti-Flag Tag Antibody pre-coated (0.2 µg/per well) plate. Serial diluted Anti-Claudin18.2 Chimeric Antibody [Zolbetuximab Biosimilar] - Azide free (A318887) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-Claudin18.2 Chimeric Antibody [Zolbetuximab Biosimilar] - Azide free (A318887) binding with Synthetic Nanodisc Human Claudin18.2 Protein (A318483) is 593.6 µg/ml.
Synthetic Nanodisc Human Claudin18.2 Protein (A318483)
Synthetic Nanodisc Human Claudin18.2 Protein (A318483) can bind Anti-Claudin18.2 Chimeric Antibody [Zolbetuximab Biosimilar] - Azide free (A318887) with an affinity constant of 1.619 nM as determined in a SPR assay.
Synthetic Nanodisc Human Claudin18.2 Protein (A318483)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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