Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human CD20 protein to be purified and analysed in vitro.
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human CD20 protein has a MW of 33.1 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA plates were added with Synthetic Nanodisc Human CD20 Protein (A318471) (0.2 µg/per well) on an Anti-Flag Tag Antibody pre-coated (0.5 µg/per well) plate. Serial diluted Anti-CD20 Humanized Antibody [Obinutuzumab Biosimilar] - Azide free (A318814) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-CD20 Monoclonal Antibody binding with Synthetic Nanodisc Human CD20 Protein (A318471) is 514.4 µg/ml.
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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