Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human CCR8 protein to be purified and analysed in vitro.
Applications
Western Blot, ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human CCR8 protein has a MW of 40.7 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
ELISA plates were pre-coated with Synthetic Nanodisc Human CCR8 Protein (A318461) (0.2 µg/well). Serial diluted Anti-CCR8 Humanized Antibody [BMS 986340] - Azide free (A318852) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From this data, the EC50 for Anti-CCR8 Monoclonal Antibody binding with CCR8-Nanodisc is 12.07 µg/ml.
Western Blot - Synthetic Nanodisc Human CCR8 Protein (A318461)
Western blot analysis of Synthetic Nanodisc Human CCR8 Protein (A318461) with Anti-CCR8 Humanized Antibody [BMS 986340] - Azide free (A318852) at 1 µg/ml, followed by Goat Anti-Human IgG Antibody (HRP) at 1:5,000 dilution.
Synthetic Nanodisc Human CCR8 Protein (A318461) can bind Anti-CCR8 Humanized Antibody [BMS 986340] - Azide free (A318852) with an affinity constant of 1.408 nM as determined in a SPR assay.
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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