Synthetic Membrane Nanoparticle Human CCR4 Protein
Description
Full-length transmembrane human CCR4 protein expressed on the cell surface of membrane nanoparticles.
Applications
ELISA, FACS
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human CCR4 protein has a MW of 41.4 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synthetic Membrane Nanoparticle Human CCR4 Protein (A318481)
ELISA plates were pre-coated with 0.5 µg/per well Synthetic Membrane Nanoparticle Human CCR4 Protein (A318481). Serial diluted Anti-CCR4 Humanized Antibody [Mogamulizumab Biosimilar] - Azide free (A318876) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-CCR4 Humanized Antibody [Mogamulizumab Biosimilar] - Azide free (A318876) binding with CCR4 full length membrane nanoparticles is 308.3 µg/ml.
Synthetic Membrane Nanoparticle Human CCR4 Protein (A318481)
FACS analysis of Synthetic Membrane Nanoparticle Human CCR4 Protein (A318481): A. Negative Control 1: Synthetic Membrane Nanoparticle Human CCR4 Protein (A318481) samples were stained only with Goat Anti-Human lgG Antibody (Alexa Fluor 488). B. Negative Control 2: Control membrane nanoparticles samples were stained with Anti-CCR4 Humanized Antibody [Mogamulizumab Biosimilar] - Azide free (A318876) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). C. Negative Control 3: Synthetic Membrane Nanoparticle Human CCR4 Protein (A318481) samples were stained with Anti-CCR8 Antibody (an irrelevant antibody) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). D. Synthetic Membrane Nanoparticle Human CCR4 Protein (A318481) samples were stained with Anti-CCR4 Humanized Antibody [Mogamulizumab Biosimilar] - Azide free (A318876) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488).
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