Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948)

Name: Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag)
Brand: Antibodies.com
SKU: A331948
Price: 590.0 USD
Availability: InStock

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$590

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Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag)

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

SARS-CoV-2

Sequence

QCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRR

Tag

C-terminal His and Avi Tag

Conjugate

Unconjugated

Biological Activity

Immobilized Recombinant SARS-CoV-2 Spike S1 (2 µg/mL) bind recombinant Human ACE2 in a functional ELISA with a linear range of 0.15-6.85 ng/mL. Immobilized Human ACE2 on COOH Chip can bind SARS-COV-2 Spike S1 in a SPR assay (Nicoya OpenSPR) with an affinity constant of 24.5 nM.

Purity

>95% (by SDS-PAGE and HPLC).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Endotoxin Level

<1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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SDS-PAGE - Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948)

SDS-PAGE of Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948) using Coomassie Blue to show a band at 110-120 kDa.

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Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948)

SEC-HPLC analysis of Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948) confirms that purity is greater than 95%.

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Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948)

Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948) binding to immobilized Human ACE2 on a COOH Chip, analyzed by surface plasmon resonance (SPR; Nicoya OpenSPR). Affinity constant = 24.5 nM.

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Standard Curve - Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948)

ELISA examination of immobilized Recombinant SARS-CoV-2 Spike S1 (2µg/mL) binding Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948): linear range = 0.15-6.85 ng/mL.

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Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948)

Shipping Information

Specifications

Scientific Validation DataValidation Data (4)

SDS-PAGE - Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948)

Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948)

Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948)

Standard Curve - Recombinant SARS-CoV-2 Spike S1 Protein (C-terminal His and Avi Tag) (A331948)