Recombinant SARS-CoV-2 Spike Glycoprotein RBD Protein (Fc Chimera 6xHis Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
SARS-CoV-2
Protein Length
Protein fragment
Sequence
S protein RBD (Arg319-Phe541) + mFc (Pro99-Lys330) + 6xHis Tag
Tag
C-terminal Mouse Fc Tag and 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 52.2 kDa after removal of the signal peptide.
Conjugate
Unconjugated
Purity
> 90%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA plates were pre-coated with Recombinant SARS-CoV-2 Spike Glycoprotein RBD Protein (Fc Chimera 6xHis Tag) (A318266) at 2 µg/ml (100 µl/well) which can bind Anti-SARS-CoV-2 S protein RBD Neutralizing Antibody (A neutralizing monoclonal antibody clone currently under clinical investigation from collaboration company) in a linear range of 0.24-9.141 ng/ml.
ELISA plates were pre-coated with Recombinant SARS-CoV-2 Spike Glycoprotein RBD Protein (Fc Chimera 6xHis Tag) (A318266) at 2 µg/ml (100 µl/well) which can bind CB6 Biosimilar - Anti-SARS-CoV-2 Spike Glycoprotein Antibody - BSA and Azide free (A318945) in a linear range of 0.24-15.62 ng/ml.
ELISA plates were pre-coated with Recombinant SARS-CoV-2 Spike Glycoprotein RBD Protein (Fc Chimera 6xHis Tag) (A318266) at 2 µg/ml (100 µl/well) which can bind Recombinant Human ACE2 Protein (Fc Tag) (A318340) in a linear range of 0.488-49.83 ng/ml.
Publishing research using Recombinant SARS-CoV-2 Spike Glycoprotein RBD Protein (Fc Chimera 6xHis Tag) (A318266)? Please let us know so that we can list the citation on this page.
