Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883)

Name: Recombinant Mouse TNF-alpha Protein (C-terminal His Tag)
Brand: Antibodies.com
SKU: A331883
Price: 190.0 USD
Availability: InStock

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$190

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Mouse TNF-alpha Protein (C-terminal His Tag)

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Mouse

Sequence

LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL

Tag

C-terminal His Tag

Conjugate

Unconjugated

Biological Activity

NF-?B reporter assay in HEK293 cells: ED50 = 40-200 pg/mL. Immobilized Mouse TNF-a (2µg/mL) binds Mouse TNFRSF1B in a functional ELISA with a linear range of 0.02-0.77ng/mL. Cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D: ED50 = 0.025-0.1 pg/mL.

Purity

>87% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Endotoxin Level

<0.1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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SDS-PAGE - Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883)

SDS-PAGE of Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883) using Coomassie Blue to show a band at 18 kDa.

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Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883)

SEC-HPLC analysis of Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883) confirms that purity is greater than 95%.

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Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883)

Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883) was measured by NF-?B reporter assay in HEK293 cells : ED50 = 40-200 pg/mL.

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Standard Curve - Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883)

ELISA examination of immobilized recombinant Mouse TNFa (2 µg/mL) binding Recombinant Mouse TNF-alpha Protein (C-terminal His Tag) (A331883): linear range = 4-53 ng/mL.

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