Recombinant Mouse IL-1 beta Protein (C-terminal His Tag) (A330795)

Name: Recombinant Mouse IL-1 beta Protein (C-terminal His Tag)
Brand: Antibodies.com
SKU: A330795
Price: 245.0 USD
Availability: InStock

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$245

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Mouse IL-1 beta Protein (C-terminal His Tag)

Expression System

Escherichia coli

Nature

Recombinant

Protein Species

Mouse

Sequence

VPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVAL GLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNW YISTSQAEHKPVFLGNNSGQDIIDFTMESVSS

Tag

C-terminal His Tag

Conjugate

Unconjugated

Biological Activity

Immobilized Mouse IL1B (10 µg/mL) bind Human IL1R2 in a functional ELISA with a linear range of 0.03125-0.8036 µg/mL. Cell proliferation assay using D10.G4.1 mouse helper T cells: ED50 = 42.1-168.4 pg/mL; Specific activity =5.94×10^6-2.37×10^7units/mg.

Purity

>95% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Endotoxin Level

<1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

Catabolin, IL1B, IL1F2, Interleukin-1 beta

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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SDS-PAGE - Recombinant Mouse IL-1 beta Protein (C-terminal His Tag) (A330795)

SDS-PAGE of Recombinant Mouse IL-1 beta Protein (C-terminal His Tag) (A330795) using Coomassie Blue to show a band at showing bands at 19-20 kDa.

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Standard Curve - Recombinant Mouse IL-1 beta Protein (C-terminal His Tag) (A330795)

ELISA examination of immobilized Mouse IL1B (10 µg/mL) binding Recombinant Mouse IL-1 beta Protein (C-terminal His Tag) (A330795): linear range = 0.03125-0.8036 µg/mL.

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Recombinant Mouse IL-1 beta Protein (C-terminal His Tag) (A330795)

Cell proliferation assay using Recombinant Mouse IL-1 beta Protein (C-terminal His Tag) (A330795) on D10.G4.1 Mouse helper T cells : ED50 = 3.10-12.40 pg/mL; Specific activity = 8.06×10^7~3.23×10^8 units/mg..

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