The biological activity was determined by measuring the dose dependent phosphorylation of ERK1 and ERK2 in CCR3 transfected 293 cells. Significant ERK phosphorylation is observed with >100 ng/ml (corresponding to a Specific Activity of 10,000 IU/mg) of recombinant mouse eotaxin.
Purity
>95% as determined by RP-HPLC and SDS-PAGE.
Product Form
Lyophilized
Concentration
1 mg/ml
Formulation
Lyophilized from a solution without additives.
Storage
Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.
