Recombinant Human VEGFA Protein (N-terminal His Tag) (A331416)

Name: Recombinant Human VEGFA Protein (N-terminal His Tag)
Brand: Antibodies.com
SKU: A331416
Price: 180.0 USD
Availability: InStock

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$180

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Human VEGFA Protein (N-terminal His Tag)

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Sequence

APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKCDKPRR

Tag

N-terminal His Tag

Conjugate

Unconjugated

Biological Activity

Immobilized Recombinant Human VEGF121 (1 µg/mL) bind Recombinant Human VEGFR2 in a functional ELISA with a linear range of 4-10 ng/mL. Cell proliferation assay using HUVEC human umbilical vein endothelial cells: ED50 = 0.09-0.36 ng/mL. Recombinant Human VEGFA(40 ng/mL) and bFGF(50 ng/mL, Cat. RP01162) induce mesoderm cells to differentiate into hematopoietic stem and progenitor cells: pebbly-like CD43+ hematopoietic stem and progenitor cells appeared in the hematogenic endothelium after 4 days.

Purity

>95% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Endotoxin Level

<0.1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

L-VEGF, Vascular endothelial growth factor A, long form, Vascular permeability factor, VEGF, VPF

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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SDS-PAGE - Recombinant Human VEGFA Protein (N-terminal His Tag) (A331416)

SDS-PAGE of Recombinant Human VEGFA Protein (N-terminal His Tag) (A331416) under reducing (R) and non-reducing (NR) conditions to show single bands at 17-22 kDa and 35-45 kDa, respectively.

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Standard Curve - Recombinant Human VEGFA Protein (N-terminal His Tag) (A331416)

ELISA examination of immobilized Recombinant Human VEGF121 (1 µg/mL) binding Recombinant Human VEGFA Protein (N-terminal His Tag) (A331416): linear range = 4-10 ng/mL.

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Recombinant Human VEGFA Protein (N-terminal His Tag) (A331416)

Cell proliferation assay using Recombinant Human VEGFA Protein (N-terminal His Tag) (A331416) on HUVEC Human umbilical vein endothelial cells : ED50 = 0.09-0.36 ng/mL.

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