Immobilized Recombinant Human VEGF165 (1 µg/mL) bind Recombinant Human VEGFR2 in a functional ELISA with a linear range of 8-20 ng/mL. Immobilized Human VEGF165 (2 µg/mL) bind Human KDR in a functional ELISA with a linear range of 0.2-11.6 ng/mL.
Purity
>97% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
L-VEGF, Vascular endothelial growth factor A, long form, Vascular permeability factor, VEGF, VPF
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Recombinant Human VEGFA Protein (N-terminal His Tag) (A331414)
SDS-PAGE of Recombinant Human VEGFA Protein (N-terminal His Tag) (A331414) under reducing (R) and non-reducing (NR) conditions to show single bands at 24-26 kDa and 44-52 kDa, respectively.
Standard Curve - Recombinant Human VEGFA Protein (N-terminal His Tag) (A331414)
ELISA examination of immobilized Recombinant Human VEGF165 (1 µg/mL) binding Recombinant Human VEGFA Protein (N-terminal His Tag) (A331414): linear range = 8-20 ng/mL.
Standard Curve - Recombinant Human VEGFA Protein (N-terminal His Tag) (A331414)
ELISA examination of immobilized Recombinant Human VEGF165 (2 µg/mL) binding Recombinant Human VEGFA Protein (N-terminal His Tag) (A331414): linear range = 0.2-11.6 ng/mL.
Recombinant Human VEGFA Protein (N-terminal His Tag) (A331414)
Cell proliferation assay using Recombinant Human VEGFA Protein (N-terminal His Tag) (A331414) on Human umbilical vein endothelial cells (HUVEC) : ED50 = typically 0.19-0.78 ng/mL; Specific activity = 1.28 × 10^6~5.26× 10^6 units/mg.
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