Recombinant Human VEGFA Protein (A331413)

Name: Recombinant Human VEGFA Protein
Brand: Antibodies.com
SKU: A331413
Price: 190.0 USD
Availability: InStock

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$190

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Human VEGFA Protein

Expression System

Escherichia coli

Nature

Recombinant

Protein Species

Human

Sequence

APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENCDKPRR

Conjugate

Unconjugated

Biological Activity

Immobilized Human VEGF121 (2 µg/mL) bind Human KDR in a functional ELISA with a linear range of 0.2-10 ng/mL. Cell proliferation assay using human umbilical vein endothelial cells (HUVEC): ED50 = typically 0.017-0.068 ng/mL; Specific activity =1.47 ×10^7-5.88 ×10^7units/mg.

Purity

>97% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from 20mM Tris, pH 8.0, with 100mM NaCl (0.22µm filter sterilized).

Endotoxin Level

<1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

L-VEGF, Vascular endothelial growth factor A, long form, Vascular permeability factor, VEGF, VPF

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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SDS-PAGE - Recombinant Human VEGFA Protein (A331413)

SDS-PAGE of Recombinant Human VEGFA Protein (A331413) using Coomassie Blue to show a band at 18 kDa.

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Standard Curve - Recombinant Human VEGFA Protein (A331413)

ELISA examination of immobilized Recombinant Human VEGF121 (2 µg/mL) binding Recombinant Human VEGFA Protein (A331413): linear range = 0.2-10 ng/mL.

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Recombinant Human VEGFA Protein (A331413)

Cell proliferation assay using Recombinant Human VEGFA Protein (A331413) on Human umbilical vein endothelial cells (HUVEC) : ED50 = typically 0.017-0.068 ng/mL; Specific activity = 1.47 ×10^7-5.88 ×10^7units/mg.

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