Recombinant Human TNF alpha Protein (C-terminal His Tag) (A331340)

Name: Recombinant Human TNF alpha Protein (C-terminal His Tag)
Brand: Antibodies.com
SKU: A331340
Price: 110.0 USD
Availability: InStock

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$110

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Human TNF alpha Protein (C-terminal His Tag)

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Sequence

VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

Tag

C-terminal His Tag

Conjugate

Unconjugated

Biological Activity

Cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D: ED50 = typically 3.46-13.84 pg/mL; Specific activity = 7.23×10^7~2.89×10^8 units/mg. Recombinant human TNF-a (20ng/mL) used to treat HeLa cells in a western-blot: level of phosphorylated p65 increased within 10 min and then decreased rapidly, indicating successful activation of NF-?B.

Purity

>95% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Endotoxin Level

<0.1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

Cachectin, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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SDS-PAGE - Recombinant Human TNF alpha Protein (C-terminal His Tag) (A331340)

SDS-PAGE of Recombinant Human TNF alpha Protein (C-terminal His Tag) (A331340) using Coomassie Blue to show a band at 18 kDa.

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Recombinant Human TNF alpha Protein (C-terminal His Tag) (A331340)

Cytotoxicity effects induced by Recombinant Human TNF alpha Protein (C-terminal His Tag) (A331340) in L-929 Mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D: ED50 = 0.90-3.60 ng/mL; Specific activity = 2.78×10^5~1.11×10^6 units/mg.

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