Recombinant Human TGF beta 1 Protein (N-terminal His Tag) (A331312)

Name: Recombinant Human TGF beta 1 Protein (N-terminal His Tag)
Brand: Antibodies.com
SKU: A331312
Price: 190.0 USD
Availability: InStock

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$190

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Human TGF beta 1 Protein (N-terminal His Tag)

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Sequence

LSTSKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Tag

N-terminal His Tag

Conjugate

Unconjugated

Biological Activity

Inhibition of the cell proliferation of TF-1 human erythroleukemic cells: ED50 = typically 0.5-2 ng/mL; Specific activity =5.0×10^5~2.0×10^6 units/mg. Immobilized recombinant human TGFB1 at (2 µg/mL) bind recombinant human TGFBR2 in a functional ELISA with a linear range of 1-5 ng/mL. Inhibition of the the IL-4-dependent proliferation of HT-2 mouse T cells: ED50 = 9.75-39 ng/mL; Specific activity =2.6×10^4~1.0×10^5 units/mg.

Purity

>95% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Endotoxin Level

<0.1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

TGFB, TGFB1, Transforming growth factor beta-1 proprotein

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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SDS-PAGE - Recombinant Human TGF beta 1 Protein (N-terminal His Tag) (A331312)

SDS-PAGE of Recombinant Human TGF beta 1 Protein (N-terminal His Tag) (A331312) using Coomassie Blue to show a band at 13 and 42 and 60kDa.

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Recombinant Human TGF beta 1 Protein (N-terminal His Tag) (A331312)

Inhibition of cell proliferation for TF-1 Human erythroleukemic cells with Recombinant Human TGF beta 1 Protein (N-terminal His Tag) (A331312): ED50 = typically 0.5-2 ng/mL; Specific activity = 5.0×10^5~2.0×10^6 units/mg.

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Standard Curve - Recombinant Human TGF beta 1 Protein (N-terminal His Tag) (A331312)

ELISA examination of immobilized recombinant human TGFB1 (2 µg/mL) binding Recombinant Human TGF beta 1 Protein (N-terminal His Tag) (A331312): linear range = 1-5 ng/mL.

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Recombinant Human TGF beta 1 Protein (N-terminal His Tag) (A331312)

Inhibition of IL-4-dependent cell proliferation for HT-2 mouse T cells with Recombinant Human TGF beta 1 Protein (N-terminal His Tag) (A331312): ED50 = 9.75-39 ng/mL; Specific activity = 2.6×10^4~1.0×10^5 units/mg.

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