Recombinant Human TGF beta 1 Protein (A331313)

Name: Recombinant Human TGF beta 1 Protein
Brand: Antibodies.com
SKU: A331313
Price: 190.0 USD
Availability: InStock

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$190

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Human TGF beta 1 Protein

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Sequence

LSTSKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Conjugate

Unconjugated

Biological Activity

Immobilized Human TGF-beta 1 (2 µg/mL) bind Human TGFBR2 in a functional ELISA with a linear range of 0.78-11 ng/mL. Inhibition of the the IL-4-dependent proliferation of HT-2 mouse T cells: ED50 = 12.85-51.40 pg/mL; Specific activity =1.95×10^7~7.78×10^7 units/mg. Cell proliferation assay using Mv.1.lu cells: ED50 = 0.08-0.32ng/mL; Specific activity =3.13×10^6~1.25×10^7 units/mg.

Purity

>95% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from 50mM Glycine, pH 3.5, with 150mM NaCl (0.22µm filter sterilized).

Endotoxin Level

<0.1 EU/µg.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

TGFB, TGFB1, Transforming growth factor beta-1 proprotein

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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SDS-PAGE - Recombinant Human TGF beta 1 Protein (A331313)

SDS-PAGE of Recombinant Human TGF beta 1 Protein (A331313) under reducing (R) and non-reducing (NR) conditions to show bands at 15 kDa and 25-30 kDa, respectively.

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Standard Curve - Recombinant Human TGF beta 1 Protein (A331313)

ELISA examination of immobilized Human TGF-beta 1 (2 µg/mL) binding Recombinant Human TGF beta 1 Protein (A331313): linear range = 0.78-11 ng/mL.

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Recombinant Human TGF beta 1 Protein (A331313)

Inhibition of IL-4-dependent cell proliferation for HT-2 mouse T cells with Recombinant Human TGF beta 1 Protein (A331313): ED50 = 12.85-51.40 pg/mL; Specific activity = 1.95×10^7~7.78×10^7 units/mg.

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