Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399)

Name: Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag)
Brand: Antibodies.com
SKU: A318399
Price: 495.0 USD
Availability: InStock

Enlarge Image

$495

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-8 business days.

Specifications

Name

Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag)

Applications

ELISA, SDS-PAGE, Flow Cytometry

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Protein Length

Protein fragment

Sequence

SIRPa (Glu31-Tyr373) + hFc (Glu99-Ala330) + 6xHis Tag

Tag

C-terminal Human Fc Tag and 6xHis Tag

Molecular Weight

The protein has a predicted molecular mass of 70-98 kDa after removal of the signal peptide.

Conjugate

Unconjugated

Purity

> 95%, by SDS-PAGE and Coomassie blue staining.

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Synonyms

BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MFR, MyD-1 antigen, MYD1, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, SIRP, SIRPA, Tyrosine-protein phosphatase non-receptor type substrate 1

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

Enlarge Image

SDS-PAGE - Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399)

SDS-PAGE of Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399) under reducing conditions.

Enlarge Image

Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399)

ELISA plates were pre-coated with Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400) at 2 µg/ml (100 µl/well) which can bind Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399) in a linear range of 3.3-26.37 ng/ml.

Enlarge Image

Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399)

A: Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399) without freeze-thaw treatment. B: Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399) after one freeze-thaw cycle. C: Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399) after three freeze-thaw cycles. D: Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399) after five freeze-thaw cycles.

Enlarge Image

Western Blot - Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399)

Western Blot analysis of Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399). Lane 1: A318399 without freeze-thaw treatment, Lane 2: A318399 after one freeze-thaw cycle, Lane 3: A318399 after three freeze-thaw cycles, Lane 4: A318399 after five freeze-thaw cycles.

Publishing research using Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399)? Please let us know so that we can list the citation on this page.