The protein has a predicted molecular mass of 47.2 kDa after removal of the signal peptide. The apparent molecular mass of SCF-hFc is approximately 55-75 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
ELISA plates were pre-coated with 1 µg/ml (100 µl/well) Recombinant Human c-Kit Protein (6×His Tag) (A318217) which can bind Recombinant Human SCF Protein (Fc Tag) (A318174) in a linear range of 3.2-400 µg/ml.
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