Lyophilized from Phosphate Buffered Saline, pH 7.4, with 8% Trehalose (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Recombinant Human PVRL1 Protein (Biotin) (C-terminal His and Avi Tag) (A331202)
Tris-Bis PAGE of Recombinant Human PVRL1 Protein (Biotin) (C-terminal His and Avi Tag) (A331202) under reducing conditions confirms that purity is greater than 95%.
Standard Curve - Recombinant Human PVRL1 Protein (Biotin) (C-terminal His and Avi Tag) (A331202)
ELISA examination of immobilized Human Nectin-3, hFc Tag, (5 µg/mL) binding Recombinant Human PVRL1 Protein (Biotin) (C-terminal His and Avi Tag) (A331202): EC50 =0.52 µg/mL.
Recombinant Human PVRL1 Protein (Biotin) (C-terminal His and Avi Tag) (A331202)
Recombinant Human PVRL1 Protein (Biotin) (C-terminal His and Avi Tag) (A331202) binding to Human Nectin-4 (Human Fc Tag) on a CM5 Chip via Protein A, analyzed by surface plasmon resonance (SPR; Biacore T200). Affinity constant = 22.83 nM.
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