This protein is constructed from the following PLA2R sequences: (Glu21-Lys164) (Thr223-Asn359) (Glu1097-Leu1246).
Applications
SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment
Sequence
PLA2R1 (Glu21-Lys164 Thr223-Asn359 Glu1097-Leu1246) + 10xHis Tag
Tag
C-terminal 10xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 50.3 kDa after removal of the signal peptide. The apparent molecular mass of PLA2R1(21-164 223-359 1097-1246)-10xHis is approximately 35-70 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 85%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.