Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318383)

Name: Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag)
Brand: Antibodies.com
SKU: A318383
Price: 585.0 USD
Availability: InStock

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This product is discontinued

Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318383) has been discontinued and is no longer available.

View all PD1 Proteins.

Specifications

Name

Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag)

Applications

ELISA, SDS-PAGE, Flow Cytometry

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Protein Length

Protein fragment.

Sequence

PD-1 (Phe24-Val170) + mFc (Pro99-Lys330) + 6xHis Tag

Tag

C-terminal Mouse Fc Tag and 6xHis Tag

Molecular Weight

The protein has a predicted molecular mass of 43.5 kDa after removal of the signal peptide.

Conjugate

Unconjugated

Purity

> 95% by SDS-PAGE and Coomassie blue staining.

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Synonyms

CD279, PDCD1, Programmed cell death protein 1, Protein PD-1

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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SDS-PAGE - Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318383)

SDS-PAGE of Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318383) under reducing conditions.

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Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318383)

ELISA plates were pre-coated with Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318383) at 2 µg/ml (100 µl/well) which can bind Pembrolizumab Biosimilar - Anti-PD1 Antibody - BSA and Azide free (A318950) in a linear range of 0.24-6.49 ng/ml.

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Flow Cytometry - Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318383)

Flow cytometry analysis of 2µg/ml of Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318383) on Expi293 cells transfected with human PD-L1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

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Flow Cytometry - Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318383)

HEK293 cell line was transfected with: irrelevant protein (red histogram) and human PD-L2 protein (blue histogram) were surface stained with 2µg/ml Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318383) followed by Goat Anti-Mouse IgG (PE) Antibody.