Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag)
Applications
ELISA, SDS-PAGE, Flow Cytometry
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment
Sequence
PD-1 (Leu25-Gln167) + hFc (Glu99-Ala330) + 6xHis Tag
Tag
C-terminal Human Fc Tag and 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 42.9 kDa after removal of the signal peptide.
Conjugate
Unconjugated
Purity
> 90%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
CD279, PDCD1, Programmed cell death protein 1, Protein PD-1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318264)
ELISA plates were pre-coated with Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318264) at 2 µg/ml (100 µl/well) which can bind Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385) in a linear range of 62.5-251.1 ng/ml.
Flow Cytometry - Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318264)
Flow cytometry analysis of 0.2µg/ml of Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318264) on Expi293 cells transfected with human PD-L1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Flow Cytometry - Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318264)
HEK293 cell line was transfected with: irrelevant protein (red histogram) and human PD-L2 protein (blue histogram) were surface stained with 2µg/ml Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318264) followed by Goat Anti-Human IgG (PE) Antibody.
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