Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment
Sequence
PD-L1 (Phe19-Arg238) + mFc (Pro99-Lys330) + 6xHis Tag
Tag
C-terminal Mouse Fc Tag and 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385)
ELISA plates were pre-coated with Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385) at 2 µg/ml (100 µl/well) which can bind Atezolizumab Biosimilar - Anti-PD-L1 Antibody - BSA and Azide free (A318947) in a linear range of 0.24-7.81 ng/ml.
Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385)
ELISA plates were pre-coated with Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318264) at 2 µg/ml (100 µl/well) which can bind Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385) in a linear range of 62.5-251.1 ng/ml.
Flow Cytometry - Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385)
Flow cytometry analysis of 15µg/ml of Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385) on Expi293 cells transfected with human PD1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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