Recombinant Human MMP2 Protein (C-terminal His Tag)
Applications
SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Tag
C-terminal His Tag
Predicted MW
71 kDa
Biologically Active
Yes
Biological Activity
The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RND,Catalog # ES001)., The specific activity is >1,000 pmoles/min/µg.Recombinant Human MMP-2 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).Activation Protocol:1. Dilute MMP2 to 100µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP2 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37°C for 1 hour.
Purity
>95% as determined by SDS-PAGE.
Product Form
Liquid
Concentration
Lot Specific
Formulation
Supplied in 20 mM Tris HCl Buffer, pH 7.4, with 150 mM Sodium Chloride and 0.05% Brij 35.
Storage
Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C. Avoid freeze/thaw cycles.
Synonyms
72 kDa gelatinase, 72 kDa type IV collagenase, CLG4A, Enamel metalloproteinase, Enamelysin, Gelatinase A, Matrix metalloproteinase-2, Matrix metalloproteinase-20, MMP-2, MMP-20, MMP20, TBE-1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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