ED50: 88-132 ng/ml, as determined by human PBMCs cultured with 0 to 1000 ng/ml Human MIF. Production of IL-8 was measured via ELISA after 24 hours.
Purity
>97% as determined by RP-HPLC and SDS-PAGE.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 10 mM Phosphate Buffered Saline, pH 7.5.
Storage
Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.
