Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Recombinant Human Mesothelin Protein (Biotin) (C-terminal Human Fc and Avi Tag) (A331050)
Tris-Bis PAGE of Recombinant Human Mesothelin Protein (Biotin) (C-terminal Human Fc and Avi Tag) (A331050) under reducing conditions confirms that purity is greater than 95%.
Recombinant Human Mesothelin Protein (Biotin) (C-terminal Human Fc and Avi Tag) (A331050)
SEC-HPLC analysis of Recombinant Human Mesothelin Protein (Biotin) (C-terminal Human Fc and Avi Tag) (A331050) confirms that purity is greater than 95%.
Flow Cytometry - Recombinant Human Mesothelin Protein (Biotin) (C-terminal Human Fc and Avi Tag) (A331050)
Expression rate of Anti-MSLN-CAR positive cell detected by Recombinant Human Mesothelin Protein (Biotin) (C-terminal Human Fc and Avi Tag) (A331050). 293T cells and anti-MSLN CAR-293T cells incubated with Recombinant Human Mesothelin Protein (Biotin) (C-terminal Human Fc and Avi Tag) (A331050). Negative control: non-transfected 293T cells and Fc-labeled protein control. SA-FITC used to evaluate the binding activity of Recombinant Human Mesothelin Protein (Biotin) (C-terminal Human Fc and Avi Tag) (A331050).
Standard Curve - Recombinant Human Mesothelin Protein (Biotin) (C-terminal Human Fc and Avi Tag) (A331050)
ELISA examination of immobilized Anti-MSLN Antibody, hFc Tag, (1µg/mL) binding Recombinant Human Mesothelin Protein (Biotin) (C-terminal Human Fc and Avi Tag) (A331050): EC50 =7.7ng/ml.
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