The protein has a predicted molecular mass of 39.3 kDa after removal of the signal peptide. The apparent molecular mass of IL5-hFc is approximately 35-55 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Flow Cytometry - Recombinant Human IL-5 Protein (Fc Tag) (A318201)
Flow cytometry analysis with Recombinant Human IL-5 Protein (Fc Tag) (A318201) at 15 µg/ml on Expi293 cells transfected with Human IL5RA protein (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant Human IL-5 Protein (Fc Tag) (A318201) which can bind Anti-IL-5 Chimeric Antibody [DMC274] - Azide free (A318784) in a linear range of 0.64-16 µg/ml.
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