The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide. The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Flow Cytometry - Recombinant Human IL-5 Protein (6xHis Tag) (A318331)
Flow cytometry analysis of 1µg/ml of Recombinant Human IL-5 Protein (6×His Tag) (A318331) on Expi293 cells transfected with Human IL5RA protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Recombinant Human IL-5 Protein (6xHis Tag) (A318331)
ELISA plates were pre-coated with Recombinant Human IL-5 Protein (6×His Tag) (A318331) at 5 µg/ml (100 µl/well) which can bind Recombinant Human IL-5RA Protein (Fc Tag) (A318202) in a linear range of 6.4-4000 ng/ml.
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