The protein has a predicted molecular mass of 41.5 kDa after removal of the signal peptide. The apparent molecular mass of IL2-mFc is approximately 35-55 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Flow Cytometry - Recombinant Human IL-2 Protein (Fc Tag) (A318165)
Flow cytometry analysis of 1µg/ml of Recombinant Human IL-2 Protein (Fc Tag) (A318165) on Expi293 cells transfected with human IL2RB (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
ELISA plates were pre-coated with Recombinant Human IL-2 Protein (Fc Tag) (A318165) at 2 µg/ml (100 µl/well) which can bind Anti-IL-2 Antibody [DM187] - BSA and Azide free (A318522) in a linear range of 0.13-3.20 ng/ml.
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