Recombinant Human IL-18 Protein (A330840)

Name: Recombinant Human IL-18 Protein
Brand: Antibodies.com
SKU: A330840
Price: 165.0 USD
Availability: InStock

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$165

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Human IL-18 Protein

Expression System

Escherichia coli

Nature

Recombinant

Protein Species

Human

Sequence

YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

Conjugate

Unconjugated

Biological Activity

Immobilized recombinant Human IL18 (5 µg/mL) bind recombinant Human IL18BPa in a functional ELISA with a linear range of 0.156-1.372 µg/mL. Induction of the IFN-gamma secretion by KG 1 human acute myelogenous leukemia cells in the presence of TNF-alpha: ED50 = 1.44-5.76 ng/mL; Specific activity =1.7×10^5~6.9×10^5 units/mg.

Purity

>95% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Endotoxin Level

<0.1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

Iboctadekin, IFN-gamma-inducing factor, IGIF, IL-1 gamma, IL18, IL1F4, Interferon gamma-inducing factor, Interleukin-1 gamma, Interleukin-18

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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SDS-PAGE - Recombinant Human IL-18 Protein (A330840)

SDS-PAGE of Recombinant Human IL-18 Protein (A330840) using Coomassie Blue to show a band at 18 kDa.

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Standard Curve - Recombinant Human IL-18 Protein (A330840)

ELISA examination of immobilized recombinant Human IL18 (5µg/mL) binding Recombinant Human IL-18 Protein (A330840): linear range = 0.156-1.372µg/mL.

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Standard Curve - Recombinant Human IL-18 Protein (A330840)

ELISA examination of Recombinant Human IL-18 Protein (A330840) to induce IFN-gamma secretion by KG1 human acute myelogenous leukemia cells in the presence of TNF-alpha: ED50 = 1.44-5.76 ng/mL; Specific activity = 1.7×10^5~6.9×10^5 units/mg.

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