Recombinant Human IL-1 beta Protein (C-terminal His Tag) (A330797)

Name: Recombinant Human IL-1 beta Protein (C-terminal His Tag)
Brand: Antibodies.com
SKU: A330797
Price: 190.0 USD
Availability: InStock

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$190

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Human IL-1 beta Protein (C-terminal His Tag)

Expression System

Escherichia coli

Nature

Recombinant

Protein Species

Human

Sequence

APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS

Tag

C-terminal His Tag

Conjugate

Unconjugated

Biological Activity

NF-?B reporter assay in HEK293 cells: ED50 = 1-10 pg/mL; Specific activity = 1×10^8~1×10^9 units/mg. Cell proliferation assay using D10.G4.1 mouse helper T cells: ED50 = 0.217-0.870ng/mL; Specific activity = 1.15×10^6~4.61×10^6 units/mg. Immobilized Human IL-1 beta (10µg/mL) bind Biotinylated Human IL-1R1 in a functional ELISA with a linear range of 0.002-1µg/mL.

Purity

>95% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Endotoxin Level

<0.1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

Catabolin, IL1B, IL1F2, Interleukin-1 beta

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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SDS-PAGE - Recombinant Human IL-1 beta Protein (C-terminal His Tag) (A330797)

SDS-PAGE of Recombinant Human IL-1 beta Protein (C-terminal His Tag) (A330797) using Coomassie Blue to show a band at 18 kDa.

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Recombinant Human IL-1 beta Protein (C-terminal His Tag) (A330797)

Recombinant Human IL-1 beta Protein (C-terminal His Tag) (A330797) was measured by NF-?B reporter assay in HEK293 cells: ED50 = 1-10 pg/mL; Specific activity = 1×10^8~1×10^9 units/mg.

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Standard Curve - Recombinant Human IL-1 beta Protein (C-terminal His Tag) (A330797)

ELISA examination of immobilized Human IL-1 beta (10 µg/mL) binding Recombinant Human IL-1 beta Protein (C-terminal His Tag) (A330797): linear range = 0.002-1 µg/mL.

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Recombinant Human IL-1 beta Protein (C-terminal His Tag) (A330797)

Cell proliferation assay using Recombinant Human IL-1 beta Protein (C-terminal His Tag) (A330797) on D10.G4.1 Mouse helper T cells : ED50 = 6.04-24.16 pg/mL; Specific activity = 4.14×10^7~1.66×10^8 units/mg.

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