Was measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The recommended concentration in this application for this effect is typically 1-5 µg/cm2. Fibronectin can also be added to the media to support cell spreading at a concentration of 0.5-50 µg/ml. Optimal concentrations will need to be determined for individual user applications.
Purity
>95% as determined by RP-HPLC and SDS-PAGE.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 20 mM Tris HCl Buffer, pH 8, with 150 mM Sodium Chloride, 5% Trehalose, and 0.02% Tween 20.
Storage
Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C. Avoid freeze/thaw cycles.
Synonyms
CIG, Cold-insoluble globulin, FN, FN1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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