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Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621)

Tris-Bis PAGE - Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621) - Antibodies.com SEC-HPLC - Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621) - Antibodies.com Standard Curve - Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621) - Antibodies.com Flow Cytometry - Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621) - Antibodies.com
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Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag)
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Sequence
LEEKKGNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPS
Tag
C-terminal His and Avi Tag
Conjugate

Biotin

Purity
>95% (by SDS-PAGE and HPLC).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4, with 8% Trehalose (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
Epidermal growth factor receptor, ERBB, ERBB1, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Tris-Bis PAGE - Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621) - Antibodies.comEnlarge Image

Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621)

Tris-Bis PAGE of Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621) under reducing conditions confirms that purity is greater than 95%.
SEC-HPLC - Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621) - Antibodies.comEnlarge Image

Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621)

SEC-HPLC analysis of Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621) confirms that purity is greater than 95%.
Standard Curve - Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621) - Antibodies.comEnlarge Image

Standard Curve - Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621)

ELISA examination of immobilized Biotinylated Human EGFRVIII, His Tag, (0.5 µg/mL) binding Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621): EC50 =4.8ng/mL.
Flow Cytometry - Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621) - Antibodies.comEnlarge Image

Flow Cytometry - Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621)

Expression rate of Anti-EGFRVIII-CAR positive cell detected by Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621). 293T cells and anti-EGFRVIII CAR-293T cells incubated with Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621). Negative control: non-transfected 293T cells and Biotinylated protein control. SA-PE used to evaluate the binding activity of Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A331621).
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