Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A330558)

Name: Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag)
Brand: Antibodies.com
SKU: A330558
Price: 805.0 USD
Availability: InStock

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$805

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Alternative Formats and ConjugatesAlternative Formats

FITC

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag)

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Sequence

LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPS

Tag

C-terminal His and Avi Tag

Conjugate

Biotin

Purity

>95% (by SDS-PAGE and HPLC).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4, with 8% Trehalose (0.22µm filter sterilized).

Endotoxin Level

<1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

Epidermal growth factor receptor, ERBB, ERBB1, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A330558)

Tris-Bis PAGE of Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A330558) under reducing conditions confirms that purity is greater than 95%.

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Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A330558)

SEC-HPLC analysis of Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A330558) confirms that purity is greater than 95%.

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Standard Curve - Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A330558)

ELISA examination of immobilized Biotinylated Human EGFR, His Tag, (1 µg/mL) binding Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A330558): EC50 =5.5ng/mL.

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Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A330558)

Human EGF binding to Recombinant Human EGFR Protein (Biotin) (C-terminal His and Avi Tag) (A330558) on a CM5 Chip via Anti-His Antibody, analyzed by surface plasmon resonance (SPR; Biacore T200). Affinity constant = 0.62 nM.

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