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Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292)

Tris-Bis PAGE - Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292) - Antibodies.com Standard Curve - Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292) - Antibodies.com SPR Assay - Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292) - Antibodies.com SPR Assay - Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292) - Antibodies.com
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Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag)
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Sequence
NKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKP
Tag
N-terminal His and Avi Tag
Conjugate

Unconjugated

Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4, with 8% Trehalose (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
T-cell-specific surface glycoprotein CD28, TP44
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Tris-Bis PAGE - Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292) - Antibodies.comEnlarge Image

Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292)

Tris-Bis PAGE of Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292) under reducing conditions confirms that purity is greater than 95%.
Standard Curve - Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292) - Antibodies.comEnlarge Image

Standard Curve - Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292)

ELISA examination of immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag, (0.5 µg/mL) binding Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292): EC50 =18.4ng/mL.
SPR Assay - Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292) - Antibodies.comEnlarge Image

Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292)

Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292) binding to Human B7-1 (Human Fc Tag) on a CM5 Chip via Protein A, analyzed by surface plasmon resonance (SPR; Biacore T200). Affinity constant = 43.61 µM.
SPR Assay - Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292) - Antibodies.comEnlarge Image

Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292)

Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292) binding to Anti-CD28 Antibody on a Protein A Chip, analyzed by surface plasmon resonance (SPR; Biacore T200). Affinity constant = 5.15nM.
Publishing research using Recombinant Human/Cynomolgus/Rhesus-macaque CD28 Protein (N-terminal His and Avi Tag) (A330292)? Please let us know so that we can list the citation on this page.

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