Recombinant Human CTLA4 Protein (C-terminal His Tag) (A330491)

Name: Recombinant Human CTLA4 Protein (C-terminal His Tag)
Brand: Antibodies.com
SKU: A330491
Price: 125.0 USD
Availability: InStock

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$125

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Human CTLA4 Protein (C-terminal His Tag)

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Sequence

AMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSDF

Tag

C-terminal His Tag

Conjugate

Unconjugated

Biological Activity

Immobilized lpilimumab (1 µg/mL) bind Recombinant Human CTLA-4 in a functional ELISA with a linear range of 6-18 ng/mL. Immobilized PE anti-human CD152 (CTLA-4) Antibody (1µg/mL (25 µL/well) can bind Human CTLA-4 in a functional ELISA with a linear range of 0.46-2.05 ng/mL.

Purity

>90% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Endotoxin Level

<1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

CD152, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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SDS-PAGE - Recombinant Human CTLA4 Protein (C-terminal His Tag) (A330491)

SDS-PAGE of Recombinant Human CTLA4 Protein (C-terminal His Tag) (A330491) using Coomassie Blue to show a band at 25 kDa.

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Recombinant Human CTLA4 Protein (C-terminal His Tag) (A330491)

SEC-HPLC analysis of Recombinant Human CTLA4 Protein (C-terminal His Tag) (A330491) confirms that purity is greater than 90%.

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Standard Curve - Recombinant Human CTLA4 Protein (C-terminal His Tag) (A330491)

ELISA examination of immobilized Recombinant Mouse CD86 (2 µg/mL) binding Recombinant Human CTLA4 Protein (C-terminal His Tag) (A330491): linear range = 15-50 ng/mL.

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Standard Curve - Recombinant Human CTLA4 Protein (C-terminal His Tag) (A330491)

ELISA examination of immobilized lpilimumab (1 µg/mL) binding Recombinant Human CTLA4 Protein (C-terminal His Tag) (A330491): linear range = 6-18 ng/mL.

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