The protein has a predicted molecular mass of 84.1 kDa after removal of the signal peptide. The apparent molecular mass of CD73-hFc is approximately 55-100 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant Human CD73 Protein (Fc Tag) (A317962) which can bind Anti-CD73 Humanized Antibody [Uliledlimab Biosimilar] - Azide free (A318841) in a linear range of 3.66-937.50 µg/ml.
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