Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag)
Description
This protein is constructed from the following CD47 sequences: (Gln19-Gln141) (Val198-Ala207) (Ser257-Gly268). The mouse Fc tag consists of amino acids Pro99-Lys330.
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment
Sequence
CD47 (Gln19-Gln141) (Val198-Ala207) (Ser257-Gly268) + mFc (Pro99-Lys330) + 6xHis Tag
Tag
C-terminal Mouse Fc Tag and 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 60 kDa after removal of the signal peptide.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400)
ELISA plates were pre-coated with Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400) at 2 µg/ml (100 µl/well) which can bind Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399) in a linear range of 3.3-26.37 ng/ml.
Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400)
ELISA plates were pre-coated with Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400) at 2 µg/ml (100 µl/well) which can bind Magrolimab Biosimilar - Anti-CD47 Antibody - BSA and Azide free (A318955) in a linear range of 0.061-1.606 ng/ml.
Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400)
ELISA plates were pre-coated with Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400) at 1 µg/ml (100 µl/well) which can bind Magrolimab Biosimilar - Anti-CD47 Antibody - BSA and Azide free (A318907) in a linear range of 3.2-80.0 ng/ml.
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