Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment
Sequence
CD28 (Asn19-Pro152) + mFc (Pro99-Lys330) + 6xHis Tag
Tag
C-terminal Mouse Fc Tag and 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 68-72 kDa after removal of the signal peptide.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
T-cell-specific surface glycoprotein CD28, TP44
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394)
ELISA plates were pre-coated with Recombinant Human CD80 Protein (Fc Tag) (A318255) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394) in a linear range of 125-4000 ng/ml.
Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394)
ELISA plates were pre-coated with Recombinant Human CD86 Protein (Fc Tag) (A318254) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394) in a linear range of 62.5-4000 ng/ml.
Flow Cytometry - Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394)
HEK293 cell line was transfected with: Irrelevant protein (B) and human B7-2 (A). Cells were surface stained with 1µg/ml of Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394) followed by Anti-Mouse IgG Antibody (Alexa 488).
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