The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide. The apparent molecular mass of CD27-hFc is approximately 55-70 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
ELISA plates were pre-coated with Recombinant Human CD27 Protein (Fc Tag) (A318253) at 2 µg/ml (100 µl/well) which can bind Varlilumab Biosimilar - Anti-CD27 Antibody - BSA and Azide free (A318938) in a linear range of 3.2-80.0 ng/ml. In order to specifically detect Varlilumab Biosimilar - Anti-CD27 Antibody - BSA and Azide free (A318938), Mouse Anti-Human Fab Antibody was used as detection antibody.
ELISA plates were pre-coated with Recombinant Human CD27 Protein (Fc Tag) (A318253) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD70 Protein (Fc Tag & 6×His Tag) (A318368) in a linear range of 3.2-400 ng/ml.
Flow Cytometry - Recombinant Human CD27 Protein (Fc Tag) (A318253)
Flow cytometry analysis of 15µg/ml of Recombinant Human CD27 Protein (Fc Tag) (A318253) on Expi293 cells transfected with human CD70 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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