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Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219)

Tris-Bis PAGE - Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219) - Antibodies.com SEC-HPLC - Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219) - Antibodies.com Standard Curve - Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219) - Antibodies.com SPR Assay - Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219) - Antibodies.com
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag)
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Sequence
EKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTDKKNRVVEPRLSNNECSCTFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARGPTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREIGIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKNTQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFGSDDG
Tag
C-terminal His and Avi Tag
Conjugate

Biotin

Purity
>95% (by SDS-PAGE and HPLC).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4, with 8% Trehalose (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
CDw116, CSF2R, CSF2RA, CSF2RY, GM-CSF-R-alpha, GMCSFR-alpha, GMR-alpha, Granulocyte-macrophage colony-stimulating factor receptor subunit alpha
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Tris-Bis PAGE - Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219) - Antibodies.comEnlarge Image

Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219)

Tris-Bis PAGE of Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219) under reducing conditions confirms that purity is greater than 95%.
SEC-HPLC - Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219) - Antibodies.comEnlarge Image

Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219)

SEC-HPLC analysis of Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219) confirms that purity is greater than 95%.
Standard Curve - Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219) - Antibodies.comEnlarge Image

Standard Curve - Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219)

ELISA examination of immobilized Anti-GM-CSF R alpha Antibody, hFc Tag, (1 µg/mL) binding Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219): EC50 =11.6ng/mL.
SPR Assay - Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219) - Antibodies.comEnlarge Image

Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219)

Human GM-CSF binding to Recombinant Human CD116 Protein (Biotin) (C-terminal His and Avi Tag) (A330219) on a CM5 Chip via Anti-His Antibody, analyzed by surface plasmon resonance (SPR; Biacore T200). Affinity constant = 1.68 nM.
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