C-Jun is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with JNK immunoprecipitated from stimulated cells. This phosphorylation can be monitored by Western blot analysis using an antibody directed to c-Jun [pS73], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.
Purity
>95% as determined by SDS-PAGE.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized without additives.
Storage
Shipped at ambient temperature. Short term (1-2 weeks) store at 4°C. Long term store at -20°C. Avoid freeze/thaw cycles.
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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