The protein has a predicted molecular mass of 51.4 kDa after removal of the signal peptide. The apparent molecular mass of B7-H4-hFc is approximately 100-130 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
ELISA plates were pre-coated with Recombinant Human B7H4 Protein (Fc Tag) (A318359) at 1 µg/ml (100 µl/well) which can bind Anti-B7-H4 Neutralizing antibody in a linear range of 3.2-80 ng/ml.
ELISA plates were pre-coated with Recombinant Human B7H4 Protein (Fc Tag) (A318359) at 2 µg/ml (100 µl/well) which can bind AZD-8205 Biosimilar - Anti-B7H4 Antibody - BSA and Azide free (A324622) in a linear range of 3.20-80 ng/ml.
ELISA plates were pre-coated with Recombinant Human B7H4 Protein (Fc Tag) (A318359) at 2 µg/ml (100 µl/well) which can bind XMT-1660 Biosimilar - Anti-B7H4 Antibody - BSA and Azide free (A324851) in a linear range of 3.20-80 ng/ml.
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