Recombinant Human 4-1BBL Protein (Fc Tag & 6xHis Tag)
Applications
SDS-PAGE, Flow Cytometry
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment
Sequence
mFc (Pro99-Lys330) + 4-1BB Ligand (Pro52-Glu254) + 6xHis Tag
Tag
N-terminal Mouse Fc Tag and C-terminal 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 49.8 kDa after removal of the signal peptide. The apparent molecular mass of mFc-4-1BB Ligand-His is approximately 53-70 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
4-1BB ligand, TNFSF9, Tumor necrosis factor ligand superfamily member 9
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Flow Cytometry - Recombinant Human 4-1BBL Protein (Fc Tag & 6xHis Tag) (A318358)
Flow cytometry analysis with Recombinant Human 4-1BBL Protein (Fc Tag & 6×His Tag) (A318358) at 15 µg/ml on Expi293 cells transfected with human 4-1BB (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
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