Recombinant Chicken Growth Hormone G119R mutant did not bind to ovine GHR-ECD and was devoid of any Biological activity in FDC-P1 3B9 cells. However, in binding experiments that were carried out using chicken liver membranes, both ovine GH and chicken GH showed similar IC50 values in competition with 125I-ovine GH, while the IC50 of G119R mutein was 10-fold higher. These results emphasize the importance of species specificity and indicate the possibility of antagonistic activity of chGH G119R in homologous system.
Purity
>98% as determined by SEC-HPLC and SDS-PAGE.
Product Form
Lyophilized
Concentration
1 mg/ml
Formulation
Lyophilized from a solution containing 0.3% Sodium Bicarbonate, pH 8.
Storage
Shipped at ambient temperature. Lyophilized: Short term (2 weeks) store at 25°C. Long term store below -18°C. Reconstituted and filter sterilization: Short term (4 weeks) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.
