The protein has a predicted molecular mass of 42.3 kDa after removal of the signal peptide. The apparent molecular mass of dPD-1-hFc is approximately 55-70 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
CD279, PDCD1, Programmed cell death protein 1, Protein PD-1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA plates were pre-coated with Recombinant Canine PD1 Protein (Fc Tag) (A324900) at 2 µg/ml (100 µl/well) which can bind INTERVET 4F12 Biosimilar - Anti-PD1 Antibody - BSA and Azide free (A324674) in a linear range of 0.64-80 ng/ml.
ELISA plates were pre-coated with Recombinant Canine PD1 Protein (Fc Tag) (A324900) at 2 µg/ml (100 µL/well) which can bind Recombinant Canine PD-L1 Protein (Fc Tag) (A324698) in a linear range of 16-400 ng/ml.
Flow Cytometry - Recombinant Canine PD1 Protein (Fc Tag) (A324900)
Flow cytometry analysis of 1µg/ml of Recombinant Canine PD1 Protein (Fc Tag) (A324900) on Expi293 cells transfected with Canine PD-L1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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