Recombinant Canine PD1 Protein (10xHis Tag) (A324898)

Name: Recombinant Canine PD1 Protein (10xHis Tag)
Brand: Antibodies.com
SKU: A324898
Price: 685.0 USD
Availability: InStock

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This product is discontinued

Recombinant Canine PD1 Protein (10xHis Tag) (A324898) has been discontinued and is no longer available.

View all PD1 Proteins.

Specifications

Name

Recombinant Canine PD1 Protein (10xHis Tag)

Applications

SDS-PAGE, ELISA, Flow Cytometry

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Canine

Protein Length

Protein fragment.

Sequence

PD-1 (Leu25-Gly168) + 10xHis tag

Tag

C-terminal 10xHis Tag

Molecular Weight

The protein has a predicted molecular mass of 17.5 kDa after removal of the signal peptide. The apparent molecular mass of dPD-1-His is approximately 15-35 kDa due to glycosylation.

Conjugate

Unconjugated

Purity

> 85% by SDS-PAGE and Coomassie blue staining.

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Synonyms

CD279, PDCD1, Programmed cell death protein 1, Protein PD-1

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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SDS-PAGE - Recombinant Canine PD1 Protein (10xHis Tag) (A324898)

SDS-PAGE of Recombinant Canine PD1 Protein (10xHis Tag) (A324898) under reducing conditions.

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Recombinant Canine PD1 Protein (10xHis Tag) (A324898)

ELISA plates were pre-coated with Recombinant Canine PD1 Protein (10xHis Tag) (A324898) at 2 µg/ml (100 µl/well) which can bind INTERVET 4F12 Biosimilar - Anti-PD1 Antibody - BSA and Azide free (A324674) in a linear range of 0.64-80 ng/ml.

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Flow Cytometry - Recombinant Canine PD1 Protein (10xHis Tag) (A324898)

Flow cytometry analysis of 15µg/ml of Recombinant Canine PD1 Protein (10xHis Tag) (A324898) on Expi293 cells transfected with Canine PD-L1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).