Recombinant Can f 1 (1.0101) Protein (A242919)

An ELISA test was designed to prove the bond between the coated target, Recombinant Can f 1 (1.0101) Protein, and allergen-specific human plasma IgG4 antibodies of a Canis familiaris alergy positive donor. A measurable signal was subsequently generated by the addition of biotin labeled anti-human IgG4 (detection) antibodies, Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated Recombinant Can f 1 (1.0101) Protein.

Purity verification: 5 µg of Recombinant Can f 1 (1.0101) Protein with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.

Flow cytometry dot-plot staining pattern of Recombinant Can f 1 (1.0101) Protein stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using Anti-CD63 [MEM-259] (FITC) Antibody and Anti-CD203c [NP4D6] (PE) Antibody.