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Recombinant Bet v 1 (1.0101) Protein (A242914)

Flow Cytometry - Recombinant Bet v 1 (1.0101) Protein (A242913) - Antibodies.com SDS-PAGE - Recombinant Bet v 1 (1.0101) Protein (A242914) - Antibodies.com ELISA - Recombinant Bet v 1 (1.0101) Protein (A242914) - Antibodies.com
$525
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Recombinant Bet v 1 (1.0101) Protein
Applications
Flow Cytometry, ELISA
Expression System
Escherichia coli
Nature
Recombinant
Protein Species
Betula verrucosa (Silver Birch)
Sequence
DNA sequence encoding 172 amino acids was fused with a Strep Tag at the N-terminus to form this recombinant protein.
Tag
Strep Tag (N-terminus)
Predicted MW
19 kDa
Conjugate

Unconjugated

Purification
Ion exchange and affinity chromatography.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 100mM Tris Buffer, pH 8, with 150mM NaCl, and without preservatives or carriers (0.2 µm filter sterilized).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Use immediately or aliquot and store at -20°C to -80°C. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Flow Cytometry - Recombinant Bet v 1 (1.0101) Protein (A242913) - Antibodies.comEnlarge Image

Flow Cytometry - Recombinant Bet v 1 (1.0101) Protein (A242914)

Flow cytometry dot-plot staining pattern of Recombinant Bet v 1 (1.0101) Protein stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using Anti-CD63 [MEM-259] (FITC) Antibody and Anti-CD203c [NP4D6] (PE) Antibody.
SDS-PAGE - Recombinant Bet v 1 (1.0101) Protein (A242914) - Antibodies.comEnlarge Image

SDS-PAGE - Recombinant Bet v 1 (1.0101) Protein (A242914)

Purity verification: 5 µg of Recombinant Bet v 1 (1.0101) Protein with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
ELISA - Recombinant Bet v 1 (1.0101) Protein (A242914) - Antibodies.comEnlarge Image

Recombinant Bet v 1 (1.0101) Protein (A242914)

An ELISA test was designed to prove the bond between the coated target, Recombinant Bet v 1 (1.0101) Protein, and allergen-specific human plasma IgG4 antibodies of Betula verrucosa positive donor. A measurable signal was subsequently generated by the addition of Anti-Human IgG4 Antibody (Biotin), Streptavidin-HRP, and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated Recombinant Bet v 1 (1.0101) Protein.
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